Chaperonin (MSP63) complexes induce bactericidal and opsonophagocytic cross-reactive antibodies
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چکیده
Methods to identify intact multi-protein complexes can help to achieve more effective formulations but their application to OMVs can be difficult due to the hydrophobicity of membrane proteins. Our previous studies of the meningococcal porin complexes using blue native polyacrylamide gel electrophoresis (BNE) (1) allowed the detection of a 700 kDa major complex. Analysis by LC/MS-MS revealed that it is a homomeric association of meningococcal stress protein MSP63 units, a protein that was found to be expressed and immunogenic during natural meningococcal infection (2).
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تاریخ انتشار 2009